Journal: STAR Protocols

Article Title: Protocol for co-culture of murine bone marrow-derived dendritic cells with OT-I mouse CD8 + T cells to evaluate immune activation efficacy

doi: 10.1016/j.xpro.2026.104621

Figure Lengend Snippet: Purity of CD8 + T cells after magnetic isolation Representative flow cytometry analysis showing the percentage of CD8 + T cells following negative selection.

Article Snippet: CD8a FITC clone 53–6.7 (Dilution: 2 μL in 100 μL FACS buffer) , Miltenyi Biotec , Cat. no. 130-118-468.

Techniques: Isolation, Flow Cytometry, Selection

Journal: STAR Protocols

Article Title: Protocol for co-culture of murine bone marrow-derived dendritic cells with OT-I mouse CD8 + T cells to evaluate immune activation efficacy

doi: 10.1016/j.xpro.2026.104621

Figure Lengend Snippet: Antigen-specific CD8 + T cell activation after co-culture with transfected BMDCs (A) Percentages of IFN- γ -producing CD8 + T cells after 6 h of co-culture with transfected BMDCs. (B) CD8 + T cell proliferation assessed by carboxyfluorescein succinimidyl ester (CFSE) dilution after 72 h of co-culture, shown as the percentage of divided cell subsets.

Article Snippet: CD8a FITC clone 53–6.7 (Dilution: 2 μL in 100 μL FACS buffer) , Miltenyi Biotec , Cat. no. 130-118-468.

Techniques: Activation Assay, Co-Culture Assay, Transfection